equipment celis
Summary

EQUIPEMENT

CELIS facility makes available a large panel of up-to date technologies/equipments to academic , iPEPS and industrial researchers

FULLY EQUIPPED CULTURE BOXES

500m2 laboratory in L2 containment

  • Biological safety station (Hood Type II)
  • CO2 incubator
  • Room dedicated to Radioactivity
  • Cell culture small equipment (pipettes, pump, racks….)
  • Dedicated temperature-controlled space (+4,-20,-80°C)
  • Bench and space to stock
  • Access to a large stock of plastic and biological consumable
  • Access to common equipment and room (dark rooms for imaging operations, shared spaces for small equipment (centrifuge, water bath, scale) as well as large dissection rooms).

STEREOMICROSCOPE ROOM

The stereomicroscope is a special type of magnifier, often called a binocular magnifier. It is a device intended primarily for observation at low magnification with illumination from above. It is mainly used for the stereoscopic perception of small subjects in relief.

CELIS facility is equipped with two large  rooms, which are equipped with:

  • 10 stereomicroscopes 
  • Stereomicroscope equipped with camera for live imaging 
  •  laminar Hoods
  •  Equipment for sterilization instrument

CELL IMAGING

2 dark rooms dedicated for Imaging

 High Content Screening  Fluorescence Imaging: Arrayscan XTI & CellInsight.

Utilisation:

  • High-throughput fluorescence imaging.
  • Counting, morphometry, molecular screening.
  • Composite images.

Server:

  • Automated transfer of data / images.
  • Posts deported for analysis.

Confocal

Nikon Confocal A1 R HD 25

  •  2/3D imaging/analysis on cell and tissue culture models
  • Confocal and wide-field system
  • Hi-content acquisition and analysis system
  • Live-cell Imaging

Videomicroscopy

Zeiss Axio Observer 7

  • Bright field
  • Phase Contrast
  • DIC
  • Fluorescence
  • Live-cell Imaging

Zeiss Axio Observer 7

  • Bright field
  • Phase Contrast
  • Fluorescence
  • Live-cell imaging
  • FRET (Förster Resonance  Energy Transfer)
  • Colocalization

Apotome

Zeiss Axio Observer 7

  • Bright field
  • Phase Contrast
  • Fluorescence
  • Structured illumination (apotome)

Wide-field inverted fluorescence microscopes

Nikon Ti Eclipse

  • 4 colors
  • Calcium Imaging

Nikon TE 2000

  • 4 colors
  • Smart shutter

Nikon TS-100

  • Simple observation
  • Acquisition 2D

Zeiss Axio Vert A1 FL

  • 4 LED
  • Simple acquisition

 

CELL PREPARATION & CELL SEPARATION

MultiMACS™ Cell24 Separator Plus: Semi-automated cell isolation on your bench

  • Simultaneous multisample magnetic cell separation
  • Processing of high sample numbers and volumes
  • Compatible with all MACS® Cell Separation strategies

GentleMACS™ Octo Dissociator with Heaters:

The gentleMACS™ Octo Dissociator with Heaters is a benchtop instrument for the fully automated and standardized tissue dissociation or homogenization of up to eight samples.

CELL COUNTING

Automated Cell Counter : Tali and Countess

The Tali® Image-Based Cytometer is a 3-channel (bright field, green fluorescence, red fluorescence) benchtop assay platform that uses state-of-the-art optics and image analysis to perform assays for cells in suspension, including GFP and RFP expression, apoptosis, cell viability (live, dead, and total cells), cell cycle, and cell counting assays. It is compatible with a wide variety of eukaryotic cells.

The Countess™ automated cell counter is a benchtop automated cell counter that performs cell count and viability measurements using trypan blue stain.

TRANSFECTION

 Nucleofector from LONZA: 2B and 4D-Nucleofector

 

Nucleofector Technology: 
The Nucleofector Technology offers a higher transfection efficiency than other non-viral transfection methods (including traditional electroporation1) along with other significant advantages outlined below.
With its superior transfection performance, Nucleofection offers various advantages over traditional electroporation methods:
High transfection efficiencies of up to 90% for plasmid DNA and 99% for oligonucleotides, like siRNA.
Excellent preservation of the physiological status and viability of transfected cells.
Analysis of transfection results already shortly after transfection possible.
Easy to use technology, with over 650 cell-type specific protocols that have led to direct transfection success.
Transfection of a wide range of substrates, including DNA, mRNA, miRNA, siRNA, peptides or proteins.
Transfection of hard-to-transfect cells, including primary cells, stem cells, neurons and cell lines.

 

BIOCHEMISTRY

Odyssey LI-COR

Odyssey LI-COR is an infrared (IR) laser-based instrumentation that is able to detect Western blots without the need for film, a darkroom, or any chemiluminescent substrates. Using infrared fluorescent signals that are directly proportional to the amount of target protein, LI-COR Odyssey can accurately and reproducibly measure changes in protein levels via ratiometric detection. In fact, the platform is able to detect both faint and strong bands within a single image, without signal saturation.

The LI-COR imager yields very low background fluorescence, which enables detection of low-picogram targets with NIR fluorescence, as well as low abundance proteins at endogenous levels. It also enables the detection of two targets simultaneously on the same membrane, which greatly increases accuracy of quantification and provides for more robust comparison.

ChemiDoc Imager

ChemiDoc Imager offer best-in-class performance with ease of use for visible light (RGB) and far red/near infrared (FR/NIR) fluorescence and chemiluminescence detection and all general gel documentation applications.Stain-free imaging enables immediate visualization of proteins without gel staining and instant verification of protein transfer to blots.

MSD Quickplex

The instrument offers affordable access to high-performance electrochemiluminescence immunoassays.