Summary

04/07/2020 Electrophysiology

in vitro ELECTROPHYSIOLOGY

Conventional patch-clamp is used in the core facility to study channelopathies and neuronal excitability.

Cellular Models

In vitro cellular models are powerful tools for the study of brain disorders. The cellular models commonly used in the core facility are:

iPSC-derived neurons
Primary cell culture
Cell lines expressing the ion channel of interest


human IPSC-derived neurons	Primary neuronal culture

Readouts

Ion channel biophysical properties
Current density
Intrinsic membrane properties
Action potential firing and excitability
Spontaneous post-synaptic potential/current

Applications

Functional screening of channelopathies
Characterization of the maturation of neuronal culture (spiking profile and synaptic connectivity)
Phenotyping of preclinical model of diseases
Target valdiation for drug dsicovery
Exploration of the effect of drug on membrane excitablity

Highlights

Functional screening of channelopathies

Fonctional caracterisation of HCN1 mutation from epileptic encephalopathy to benign generalized epilepsy and beyond. Publication

Characterization of the maturation of neuronal culture

Fonctional characterization of the human IPSC-derived neurons in Parkinson’s disease. Publication

Services

Generation of full data set : experiments design, data acquisition and analysis
Access to the facility setup for trained electrophysiologists
Training in electrophysiology
R&D assays
Consulting

ex vivo PATCH-CLAMP

MULTIELECTRODE ARRAY